July 2003 Volume VII, No. 1NEW BOOKS, CHAPTERS, AND REVIEWS In response to the rising demand for faster, more accurate and
precise ways to determine the potentially lethal type-A trichothecene
(T-2 toxin) in cereals, researchers at the Institute of Sciences
of Food Production in Bari, Italy, tested the effectiveness of high
performance liquid chromatography (HPLC) used with commercially
available products for fluorescent labeling and sample cleanup.An
easily performed method has been developed that shows superior sensitivity,
selectivity, and repeatability of T-2 toxin measurements when compared
to other methods.Recoveries were higher than 80% and the limit of
detection was 0.005 µg/kg. Sample extracts were purified by
T-2-TAG immunoaffinity columns from VICAM .The fluorescent labeling
reagent 1-anthroylnitrile was used to make the toxin detectable
by HPLC. The method has been published in Journal of Chromatography
A 989, 257-264, 2003, and is available online at the following website: |